?33 The results found among Parsis and other classes in the north Fort is noted below :- Percentage, Class No.1. Percentage, Class No.2. Percentage, Class No.3. Percentage, Class No.4. Percentage, Class No.5. Parsis 61.3 14.1 14.8 5.0 1.2 Other Classes ... 70.2 18.5 12.2 3.2 0.7 78. BLOOD EXAMINATION.-Although the spleen census gives most valuable information regarding antecedent malaria and may be used as a measure of malarial endemicity, the microscopic examination of the blood gives informa- tion regarding the actual presence of infection, and the species of parasite, not to be gained in any other way. Years ago Stephens and Christophers suggested, as a measure of malarial endemicity, the use of an infection index arrived at by the microscopical examination of the blood of young children. This suggestion was based upon a consideration of the following facts. In a very malarious locality nearly all new comers usually contract malaria after a short stay of days or weeks ; in a less malarious locality a smaller proportion become infected in a given period; and in a place where malaria is only mild, new comers may escape infection for months or even years. Therefore the examination of new comers for the presence of malarial infection enables the observer to arrive at a figure of value for comparative purposes ; and as a rule young children are selected for the purposes of this examination because they form a class of new comers more or less fixed in character and available everywhere. But in Bombay, where a large proportion of the population is formed of new arrivals and temporary residents, there are many thousands of adults who are probably quite as susceptible to malarial infection as young children ; and it has been found that the adult population of malarious localities in the City is infected with malaria in much the same proportion as the child population. During the present enquiry a large number of blood films have been collected from both children and adults in different parts of the City. As it is quite impossible to examine the blood of more than a small proportion of the population in a City as large as Bombay, it was necessary to adopt a system of" random sampling," when obtaining specimens for the estimation of the parasite index. The method employed in the collection of blood films during the investigation was as follows:-A locality, chosen indis- criminately, was visited usually in the early morning. Conversation was exchanged with some resident or passer-by until a crowd collected. After a little persuasion, some one would consent to allow his finger to be pricked and a blood film prepared, and it would soon become fashionable to follow suit. Children coming forward or brought by their parents were rewarded with a pice. In this way it was usually possible in the course of an hour to collect 50 or 60 blood films, specimens being taken from every one in the crowd who appeared willing. Blood films were collected in this manner from over 00 localities in different parts of the City. 79. Parasite Index.-The parasite index of the different wards in the City obtained by the examination of the blood of people from about 100 different localities is as follows :- Tables of blood examination showing the amount of street infection in each ward. Ward. Number examined. Number infected. Percentage infected. Ward A. ... ... 2,681 943 34.8 ,, B. ... ... 473 65 13.7 ,, C. ... ... 707 196 27.7 ,, D. ... ... 354 19 5.3 ,, E. ... ... 878 33 3.7 ,, F. and G. ... 302 11 3.6 Total ... 5,395 1,267 23.4 B 136-9