?214 (9) To lock the carriages before the arrival of the train at the inspection station. (Paragraph 3, page 174.) (10) To detain the train until the medical inspection is over. (Paragraph 2, page 174.) (11) To see that no passenger is permitted to leave his carriage until in orderly course the arrangement shall be complete for his examination. (Paragraph 3, page 174.) (12) To give every assistance to the medical officers conducting the inspection in order to minimise the detention of trains. (Paragraph 2, page 174.) (13) To carry out the instructions regarding the trans- mission of the names and addresses of passengers. (Regulation 17 (viii), page 33; paragraph 15, page 182.) (14) To afford every facility to wards the working of the Plague Regulations generally. (G.O., No. 1165-P., dated 26th September 1898.) (6)-Plague Research Laboratory, Bombay. 1. The following rules should be observed by officers when sending fresh materials to the Research Laboratory for bacteriological examination:- A.-Dead animals, rats, &c.- (i) These should only be sent if freshly dead or at least before any signs of putrefaction are evident. (ii) If sent by post from outlying stations, they should be most carefully packed in ice, no spirit or other pre- servative fluid of any kind being used: water-tight bottles or cases being used in each case. (iii) In Bombay they ought to be sent by special messenger at once, or if found late at night, to be packed in ice and despatched early the following morning. B.-Excreta, sputa and the like- (i) These should be sent in carefully stoppered and sealed bottles. (ii) No fluid of a preservative nature ought to be added to them. C.-When any of the above articles are sent by post, the rules laid down by the Government of India in Resolution No. 1077-1086 of 9th August 1898 should be observed, viz., " great care should be taken that the stopper or cork of the bottle fits tightly." " The stopper should be carefully tied down with bladder or leather and sealed."