INSTITUTE OF PUBLIC HEALTH, RANGOON, FOR THE YEAR 1935.
5
provisions of the Burma Underground Water Act, and thirty-three such
samples were received during the year.
Eight samples of water were sent to be examined for the presence
of cholera vibrio. All were negative except one sample, from which
an organism simulating the cholera vibrio was isolated, but it did not
agglutinate with stock high titre serum.
Twenty-one samples of shaving brushes of different manufacture
were sent by the Customs Department to be examined for the presence
of anthrax bacillus; on no occasion was this organism found.
Four samples of disinfectants were tested for their carbolic acid
coefficient. Out of these, three samples were received from the Police
Supplies Depôt, Rangoon. It must be noted here that this examination
gives only a comparative result, as it is not possible to maintain
standard requirements for the Rideal Walker test in this laboratory at
present.
Bacterial emulsions of the typhoid and paratyphoid group of
organisms were prepared in accordance with instructions detailed in
the Medical Council Research Series No. 51.
Preparation of high titre agglutinating sera was started for the first
time in this laboratory this year. In previous years, such sera had to
be purchased from the Pasteur Institute of Burma.
The following stock cultures were maintained in this laboratory
during the year:—
| 1. B. typhosus. | 9. Vibrio choleræ (ten strains). |
| 2. B. paratyphosus A. | 10. Ratin virus. |
| 3. B. paratyphosus B. | 11. Ratin 116. |
| 4. B.coli. | 12. Ratin 688. |
| 5. B. dysentery Flexner. | 13. Liverpool virus. |
| 6. B. dysentery Shiga. | 14. Danyz's virus. |
| 7. B. pestis. | 15. Ps pyocyancus. |
| 8. Staphylococcus. | 16. Ch. prodigiosus. |
6. PREPARATION OF CHOLERA VACCINE.—This has turned out to
be the heaviest item in the list of duties of this laboratory. As this
laboratory did not possess the necessary apparatus, preliminary experi-
ments and the manufacture of the vaccine on a small scale were started
with the help of the Pasteur Institute of Burma, and the thanks of
the writer are due to the Director of that Institute for his co-operation.
Large scale preparation of the vaccine was started in the Harcourt
Butler Institute from the month of September when the laboratory
was equipped with the necessary apparatus.
During the year, about 80,000 doses of this vaccine were prepared,
out of which 20,000 doses, approximately, were filled in ampoules and
issued. The manufacture of this vaccine in the Institute means a
large saving to the Local Government.