8
Scientific Memoirs by
Numbe of specimen. |
Date at which procur- ed. |
Source. | Reaction. | Nature of eultivation. |
Date at which set. |
RESULT. |
XVII | July 15th. |
Bazaar | Faintly alkaline on drying. |
Plate. Inoc- ulated with 0.03 C. C. from the bot- tom of the milk. |
July 15th. |
July 16th. Crowded with colonies of common lacteal Schizomycetes in the proportion of 340,965 per C. C. Small straight Bacill and short oval ones the most prevalent forms |
XVIII | July 25th. |
„ | Neutral. | Plate. Inoc- ulated as above with 0.03 C. C. |
„ | July 26th. Smell mouldy. Abundant colonies of typical lacteal Micrococcus, oval and small straight Bacilli. |
XIX | July 29th. |
„ | „ | Plate. Inoc- ulated as above. |
July 29th. |
July 3oth. Numerous colonies in the propor- tion of 51,172 per C. C. Most of them con- sisting of the common lacteal large Micro- coccus and short oval Bacilli. A few super- ficial bluish colonies of small, straight Bacilli also present. |
XX | Aug. 15th. |
„ | Faintly alkaline. |
„ | Aug. 15th. |
August 16th. Colonies present in the propor- tion of 39,847 per C. C. Consisting of com- mon lacteal Micrococcus and small straight Bacilli. |
This table records the results of cultivations of unboiled milk. These
show that the milk in common use in Calcutta contains a varying but always
very large number of schizomycete organisms. The numbers actually present
in those cases where an estimate was made by numeration of colonies present
within determined areas of plate-cultivations ranged from a proportion of only
3,400 per C. C. of milk to over 300,000 per C. C.
Determinations of this nature were made in regard to 14 specimens with
the following results:—
Case 1 | 100,000 | Schizomycetes per C. C. of milk |
2 | 20,000 | „ „ „ „ |
3 | 48,000 | „ „ „ „ |
4 | 132,000 | „ „ „ „ |
5 | 80,000 | „ „ „ „ |
6 | 103,950 | „ „ „ „ |
7 | 51,000 | „ „ „ „ |
8 | 340,000 | „ „ „ „ |
9 | 25,245 | „ „ „ „ |
10 | 3,400 | „ „ „ „ |
11 | 8,670 | „ „ „ „ |
12 | 349,965 | „ „ „ „ |
13 | 51,172 | „ „ „ „ |
14 | 39 847 | „ „ „ „ |
One noteworthy point is that the exceptional specimen of milk procured under
special precautions, and which did not undergo the normal acid fermentation and
coagulation, was not free from schizomycetes, nor did it even contain an excep-