24

SERIES No. III.—Experiments to ascertain if the venom of Bungarus fasciatus
has the power of clotting citrate plasma in vitro, no soluble salt of lime
being added.

     Two series of experiments were made, one with citrate donkey plasma and
the other with citrate horse plasma. In each instance the citrate of soda was
present in the strength of 1 in 100 of plasma. The plasma was completely free
from red cells.

     Two cubic centimetres of plasma were measured into each tube, to each of
which there was then added a different amount of the venom of Bungarus fas-
ciatus. The strength of the venom solution used was 1 per cent. It was dis-
solved in normal saline solution.

     The following were the results obtained :—

(a) Donkey plasma (1 per cent. citrate).

Plasma. Bungarus fas-
ciatus venom in
milligrammes.		 Result.
2 c.c. . . . . . . . . . 5 Liquid after 24 hours.
2 " . . . . . . . . . 4 " " 24 "
2 " . . . . . . . . . 3 " " 24 "
2 " . . . . . . . . . 2 " " 24 "
2 " . . . . . . . . . 1 " " 24 "

(b) Horse plasma (1 per cent. citrate).

Plasma. Bungarus fas-
ciatus venom in
milligrammes.		 Result.
2 c.c. . . . . . . . . . 5 Liquid after 24 hours.
2 " . . . . . . . . . 4 " " 24 "
2 " . . . . . . . . . 3 " " 24 "
2 " . . . . . . . . . 2 " " 24 "
2 " . . . . . . . . . 1 " " 24 "

SERIES No. IV.—Experiments to ascertain if the venom of Bungarus fascia-
tus can prevent the clotting of citrate plasma, which normally results from
the addition of a soluble salt of lime.

     Two series of experiments were made, one with citrate donkey plasma and
the other with citrate horse plasma. In each case the citrate of soda was
present in the strength of 1 in 100 of plasmas.

     Two cubic centimetres of plasma were measured into each tube, to each of