24
The test dose of each poison, namely, an amount which clotted the control
in a few minutes, was mixed with varying amounts of serum. The mixtures were
allowed to stand at laboratory temperature for half an hour. Then into each
tube there were run 2 c.c. of citrate plasma. A control tube without serum was
prepared in each instance.
The following results were obtained.
Amount of Serum. |
H. C. V. ( 0.1 milligr.) |
E. C. V. ( 0.05 milligr.) |
T. G. V. ( 0.3 milligr.) |
C. A. V. ( 0.05 milligr.) |
||||
1 C.C. | Clotted in 6 minutes. |
Clotted in 5 minutes. |
Clotted in 11 minutes |
Clotted in 10 minutes. |
||||
0.5 | " | Ditto. | Ditto . | Ditto . | Ditto. | |||
C.1 | " | Ditto. | Ditto . | Ditto . | Ditto. | |||
Nil (control) | Ditto. | Ditto . | Ditto . | Ditto. |
It is evident from these experiments that C. V. serum cannot prevent the.
clotting action in vitro of any of these four venoms.
TABLE XVIII.—Experiments to ascertain if H. C. V. serum can neutralise
the actions of the venoms of (z) Hoplocephalus curtus, (2) Echis carinata,
(3) Trimeresurus giamineus and (4) Crotalus adamanteus on citrate
plasma in vitro.
The same technique was used as was employed in the observations detailed
on Table XVII. The following were the results.
Amount of serum. |
H. C. V. (0.1 milligr.) |
E. C. V. (0.05 milligr.) |
T. G. V. (0.3 milligr.) |
C. A. V. (0.05 milligr.) |
||||
1 | c.c. | Liquid after 24 hours. |
Clotted in 5 minutes. |
Clotted in 13 minutes. |
Clotted in 11 minutes. |
|||
0.5 | " | Ditto . | Ditto . | Ditto . | Ditto. | |||
0.1 | " | Ditto . | Ditto . | Ditto . | Ditto. | |||
0.08 | " | Ditto . | ... | ... | ... | |||
0.06 | " | Ditto . | ... | ... | ... | |||
0.04 | " | Trace of clot after 24 hours. |
... | ... | ... | |||
0.02 | " | Clotted after 24 hours. |
... | ... | ... | |||
0.1 | " | Clotted in 13 minutes. |
... | ... | ... | |||
Nil (Control) | Clotted in 6 minutes. |
Clotted in 5 minutes. |
Clotted in 13 minutes. |
Clotted in 11 minutes. |