24

     The test dose of each poison, namely, an amount which clotted the control
in a few minutes, was mixed with varying amounts of serum. The mixtures were
allowed to stand at laboratory temperature for half an hour. Then into each
tube there were run 2 c.c. of citrate plasma. A control tube without serum was
prepared in each instance.

     The following results were obtained.

Amount of
Serum.
H. C. V.
( 0.1 milligr.)
E. C. V.
( 0.05 milligr.)
T. G. V.
( 0.3 milligr.)
C. A. V.
( 0.05 milligr.)
1 C.C.       Clotted in
6 minutes.
Clotted in
5 minutes.
Clotted in
11 minutes
Clotted in
10 minutes.
0.5 "       Ditto. Ditto . Ditto . Ditto.
C.1 "       Ditto. Ditto . Ditto . Ditto.
Nil (control)     Ditto. Ditto . Ditto . Ditto.

     It is evident from these experiments that C. V. serum cannot prevent the.
clotting action in vitro of any of these four venoms.

TABLE XVIII.—Experiments to ascertain if H. C. V. serum can neutralise
     the actions of the venoms of (z) Hoplocephalus curtus,
(2) Echis carinata,
     
(3) Trimeresurus giamineus and (4) Crotalus adamanteus on citrate
     plasma
in vitro.

     The same technique was used as was employed in the observations detailed
on Table XVII. The following were the results.

Amount of
serum.
H. C. V.
(0.1 milligr.)
E. C. V.
(0.05 milligr.)
T. G. V.
(0.3 milligr.)
C. A. V.
(0.05 milligr.)
1 c.c.       Liquid after 24
hours.
Clotted in 5
minutes.
Clotted in 13
minutes.
Clotted in 11
minutes.
0.5 "       Ditto . Ditto . Ditto . Ditto.
0.1 "       Ditto . Ditto . Ditto . Ditto.
0.08 "       Ditto . ... ... ...
0.06 "       Ditto . ... ... ...
0.04 "       Trace of clot after
24 hours.
... ... ...
0.02 "       Clotted after 24
hours.
... ... ...
0.1 "       Clotted in 13
minutes.
... ... ...
Nil (Control)     Clotted in 6
minutes.
Clotted in 5
minutes.
Clotted in 13
minutes.
Clotted in 11
minutes.