15

      There was a well marked local reaction at the site of inoculation; this
resolved without abscess formation. The animal was never at any time as ill
as the first monkey had been; it never refused food and, in fact, appeared
always to be in fair health. For temperature see chart.

      The serum agglutination reaction with three races of M. Melitensis was
estimated on three occasions.

      First, before inoculation no reaction was obtained in dilution of 1 in 4 with
stock, Multan I or Multan II.

      Secondly, at the beginning of the third week complete sedimentation in
dilution of 1 in 40 was observed with all three strains.

      Thirdly, at the beginning of the fourth week the following result was
obtained:—

      These estimations show a great increase in the agglutinin content of the
blood in the course of one week.

      At the beginning of the fifth week 2 c.c. of blood were removed from a peri-
pheral vein and planted in broth and on agar. A pure growth of M. Melitensis
was got from one of the broth tubes. The other tubes remained sterile.

      At the end of the ninth week the animal was killed with chloroform. Cultures
were made from the heart's blood, from the liver and from the spleen. The
tubes inoculated with heart's blood remained sterile. A pure culture of M.
Melitensis was obtained from both the liver and spleen. This organism responded
to all the tests mentioned above.

      Group b.—We have now briefly to refer to a few cases of continued fever,
in which for various reasons no attempt was made to isolate the causal agent of
the disease, but the sera of which gave a good agglutination reaction with
M. Melitensis. These cases in the light of our recent researches we must regard
as undoubted cases of Mediterranean fever.

      Case XII.—Sohun, aged 32; Sepoy, 27th Punjabis, Multan. Patient
was admitted into hospital on 6th March 1905 complaining of cough and fever.