13

folds of the paper. These tubes were then placed in a small dish standing in
a basin of water. No corks are necessary if the paper is pushed well down
to the bottom as the bugs cannot climb up the glass. On taking the tubes
to the bedside, the paper containing the bugs was gently drawn up to, the
mouth of each tube with a pair of forceps and the tubes inverted over the
patient's skin, shortly after which the bugs came out and began to suck blood,
and when fully gorged returned readily to the folds. Bugs fed in this way live
and breed as in nature, and can be fed either by day or night.

   On March 28th, a large number of bugs were obtained from many sources
and were placed in a jar containing some crumpled filter paper; the next day
suitable specimens were removed with a fine pair of forceps and placed in a
tube ready for use.

   Experiment I, Case 11.—On March 29th, eleven bugs were fed on this
patient, in a film of whose finger blood nine parasites had been found. On
March 30th, 31st, and April 2nd the bugs were again fed although no parasites
had been found in blood films on those dates. On April 2nd one of the bugs was
dissected and the mid-gut contents which contained fresh and altered blood were
examined in two separate films under cover slips. In one of the preparations I
saw a pyriform-shaped flagellate actively moving about among the corpuscles.
Its structure was difficult to make out in the fresh condition, but a hyaline
nucleus situated about the centre of the body was visible. I was unable,
however, to make out the blepharoplast or any granules in the protoplasm.
Its movements were less active than those of the flagellates found in Anopheles
stephensi, its flagellum exhibiting slow lashing movements.

   The flagellates of Anopheles stephensi are extremely active, darting across
the field of the microscope, then suddenly altering their course and moving
in the opposite direction; their flagella exhibit vibratile rather than lashing move-
ments. The movements of this flagellate of the bug were more like those
of the flagellates of Culex fatigans and Culex pipiens. I was at first in doubt
where this flagellate came from, for, on examining the saline solution used in my
dissections, I could find no organisms of this nature. On attempting to make
a stained preparation the parasite was unfortunately lost, so I was unable to
study it further. Thinking that it might possibly prove to be the fully developed
Leishman-Donovan body, I determined to go on with my experiments with bugs.

   It is not necessary to give details of the dissections of 41 bugs examined
between April 2nd and 13th, as I was unable to procure a suitable case in
hospital and all the dissections proved negative.

   On April 14th I was shown by Mr. Haller a case of Kala Azar in a
European, in the last stages of the disease, suffering from very severe diarrhœa.
No blood film was examined in this case, Six bugs were removed from the