FOWL TYPHOID IN INDIA 105
It is thus not possible, as has already been reported by a number of workers, to
distinguish by the agglutination test between fowl typhoid caused by S. gallinarum
and bacillary white diarrhœa caused by S. pullorum.
Dead bacterial culture was prepared for use as a vaccine, by growing the
organism on agar plates, collecting the growth, emulsifying it in carbolised normal
saline solution to opacity 8 by barium sulphate standard tubes and heating it at
65°C. for one hour. This vaccine, however, failed entirely to protect fowls in-
oculated with it against a small dose of the living organism given by subcutaneous
injection. Details are given in Table IV.
TABLE IV.
Fowl No. |
1st vaccination |
2nd vaccination |
Test with S. gallinarum |
Result |
|||
Date |
Dose |
Date |
Dose |
Date |
Dose |
||
23A. |
29-8-30 |
1 c. c. |
Nil. |
Nil. |
Died 4th day. |
||
3A. |
Died 6th day. |
||||||
30A. |
Died 8th Day. |
||||||
53A. |
5-9-30 |
0. 5 c. c. |
Lived. |
||||
572A. |
Died 2nd day. |
||||||
585A. |
Nil. Controls. |
Died 6th day. |
|||||
586A. |
Died 6th day. |
||||||
12B. |
29-8-30 |
1 c. c. |
12-9-30 |
5 c. c. |
Died 6th day. |
||
523A. |
Died 7th day. |
||||||
588A. |
19-9-30 |
0. 5 c. c. |
Died 7th day. |
||||
15A |
Recovered. |
||||||
20A. |
Nil. |
Controls. |
Died 4th day. |
||||
571A. |
Died 22nd day. |