356 THE INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY [ I, IV
8. The virus does not affect mammals.
9. The virus is found in the spleen, kidneys, ovaries, testes, liver, lungs, crop, saliva, intestines
and brain, very rarely in the blood and the faeces, and not in the gelatinous infiltration at the thoracic
inlet.
10. Fifty degrees Centigrade to 55°C. for 30 minutes kills the virus.
11. Direct sunlight kills the virus in one hour exposure.
12. A 1 : 10000 aqueous solution of potassium permanganate inactivates the virus in 40 minutes.
13. Sheep serum has a neutralizing effect on the virus of avian pest in vitro.
14. Immune serum (l½-cuhic centimeter serum to 125 minimum infective doses) has a neutraliz-
ing effect on virus.
15. The minimum infective dose of the virus (minimum infective doses) was 1 cubic centimeter
of a dilution of 1 to 1,250 of saliva.
16. The virus of avian pest as determined by cataphoresis is negatively charged.
17. The virus is not thrown down by centrifugation.
18. Virus can be kept viable in the ice-chest for at least 10 months and 20 days ; at room tem-
perature virus in the liquid form remained active for 15 days. Virus in garden soil and in stagnant
water placed in a room where diffuse sunlight entered through rough glass was inactivated in 4
days. Active virus can be obtained from a bird 168 hours after death
19. The cross-immunity tests performed independently by Doctors Doyle, Picard, and Cooper
and those by us were unanimous in results.
20. The treatment of artificial infection by the use of drugs has so far been quite unsuc-
cessful. The result of individual treatment with Fowler's solution naturally injected is encouraging
if given early.
21. Treatment by the use of immune serum injected intravenously was partially successful.
22. The employment of immune serum as a field control measure was unsatissfactory.
23. The control of outbreaks by means of chemicals has been successful in well equipped poultry
yards.
24. Attempts to produce a vaccine of prophylactic or therapeutic value have failed. The few
good results could not be repeated.
25. Diagnosis by complement fixation was not successful. [ Reprinted from the Philippine
Journal of Agriculture, 1, 4,1930.]