150     INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY [ III, II

was associated with the pseudoglobulin fraction in the serum of horses immunised
against tetanus toxin. The same author found that typhoid agglutinins of horse
serum were contained in the pseudoglobulin fraction, while cholera lysin and
cholera agglutinin in the horse and goat occurred in the euglobulin fraction.
According to Gibson and Collins [1907] the agglutinins for the dysentery group of
organisms, typhoid, coli, and cholera, are not confined to either of the globulin
fractions. Landsteiner [1900] showed that the substance occurring in normal
serum which exerts an antitryptic effect is associated with the albumin fraction of
the serum, and this was confirmed by Cathcart [1904]. Opie and Barker [1907]
found that an anti-leucoprotcase occurs in serum albumin. The presence of an
antiviral body in antiviral serum was first demonstrated by Sternberg [1892] in an
anti-vaccinial serum, and Béclère, Chambon, and Menard [1899], in attempting to
locate these bodies, found them to be associated only with the globulin fraction of
the serum. Hartley [1914] attempted to concentrate an antiviral serum by salt-
ing out the different protein fractions. Working with anti-rinderpest serum he
found the antibodies to be exclusively associated with the euglobulin fraction and
not with the pseudoglobulin or albumin fractions, which he has shown to have no
protective powers. Henseval [1919] found that all the protein fractions of anti-
vaccinial serum including the albumin portion possessed some degree of antiviral
activity, but Findlay [1931] has recently demonstrated that the immune body
responsible for the protective action of rabbit anti-vaccinial serum is associated with
the globulin fraction, the major part of it being with the euglobulin portion and
the smaller with the pseudoglobulin. Ledingham, Morgan and Petrie [1931] have
obtained an anti-vaccinial serum by hyper-immunisation of the horse with the
virus of vaccinia, and they have found that the antiviral body is associated with
the euglobulin as well as with the pseudoglobulin fractions of the serum, but not
with albumin. In horse anti-poliomyelitis serum Morgan and Fairbrother [1930]
found the highest concentration of the antibody in the euglobulin portion although
there was a general distribution of the antibody throughout the serum proteins.
Weyer, Park and Banzhaf [1929], on the other hand, had previously located the
antibodies of anti-poliomyelitis serum in the pseudoglobulin fraction alone.
Felton [1925, 1928] in a series of experiments on anti-pneumococcus serum finds
that the major part of the protective bodies are associated with that small fraction
of globulin which occurs near the border line of the salt precipitation limits of
euglobulin and pseudoglobulin. Goodner [1930] locates the antibodies of anti
meningococcus serum in the Felton fraction of the serum, and it seems that the
distemper antibody is also associated with the same fraction [Laidlaw and Dunkin,
1931]. Mallick and Maitra [1932] have found that the antibodies of anti-venomous
horse serum are mainly associated with the pseudoglobulin fraction. It will thus