306 THE INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY [VI, III

neously into a cow, and 5 weeks afterwards when the animal was slaughtered, no changes
were discovered at the seat of inoculation or elsewhere. In two of the experimental
healthy cows, the calcium content was found to decrease and the phosphoric acid
content to increase. Further in order to compensate for the loss of blood in the urine,
a quantity of blood from a healthy cow was given intravenously to an affected animal
but no amelioration resulted. At autopsy of experimental animals, lesions of tuber-
culosis have been recorded from most animals but no explanation for this unusual
finding has been offered. In the small animal tests, 4 white mice, 3 guinea pigs
and 2 rabbits were inoculated with 1, 2 and 5 c.c. of strongly bloody urine at the root
of the tail but no reaction was noticed in life or on destruction.

From the above experiments and the failure to find any parasites in the post
mortem examination of clinically affected cases, the author concludes that the disease
is not transmissible, is not contagious or infectious, and no parasite is involved in
its causation.

Again to test whether any general poisoning through acids can set up haematuria,
commercial acids and spring water from notorious localities were tested experimentally,
by administration to healthy animals in such high proportions as neither the fodder
nor the water of enzootic areas could possibly contain remembering of course that
even if such concentrations were possible in nature, cattle would surely refuse to take
them. Increasing doses and varying concentrations of nitric acid (up to 65 c.c. of
0.17 to 0.39 per cent solutions), silicic acid (solution containing up to 50 grms. of
Potassium silicate in 0.29 to 1.06 per cent solution) and Oxalic acid (170 grms. of
Oxalic acid in 0.2 to 2.43 per cent solution) were given in drinking water over a length
of time. No changes were noticed in any of the animals. On the basis of these experi-
ments, the author concludes that these common acids do not cause haematuria in
living animals, and that the water from enzootic areas does not contain any virus or
bacteria capable of transmitting the disease. [S. C. A. D.].

L'acaprine dans le traitement de la piroplasmose bovine vraie des bovides
due a Piroplasma bigeminum (Smith et Kilborns) [Acaprine in the
Treatment of true Bovine Piroplasmosis due to Piroplasma bigeminum
(SMITH and KILBORNE) Cernaianu, C., Radef, I., & Radescu, T. (1935).
Bull. Soc. Path. Exot. 28, 804-806.

During 1934, the authors successfully used Acaprine in the treatment of twelve
bovines, the majority of which showed very severe symptoms of Babesia bigemina
infection. The protocols of three of these animals are given and from these it would
appear that in all cases a marked amelioration of the clinical symptoms resulted within
24 hours of drug injection. Four other animals, which were in a highly advanced
stage of the disease and were lying down on the ground, died within 2 to 4 hours of
drug intervention. During 1935, the authors treated fourteen further animals showing
inappetence, an icteric condition of the mucous membranes and symptoms of haemo-
globinuria, and recovery was obtained in nine of these animals as the result of a single
injection of Acaprine.