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324 THE INDIAN JOURNAL OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY [VI, IV

fed by hand. The temperature of the birds was recorded twice daily. Details
of these birds are given in Table I. The following is the abstract of the same :—

Serial No.	Fowl	Interval between the injection of trypanblue and infection	Date of injecting		Date of exposing to contact infection	Date of death	Interval between the date of expo- sure to infection and death
Serial No.	Fowl	Interval between the injection of trypanblue and infection	Trypanblue	Virus	Date of exposing to contact infection	Date of death
1	Cock . .	One week.	19th Jan., 1936.	26th Jan., 1936.		6th 1936. Feb.,	11 days.
2	Hen . .		Do. .	Do. .		30th 1936. Jan.,	4 „
3	Hen . .		Do. .	Do. .		31st 1936. Jan.,	5 „
4	Hen . .		Do. .	Nil .	26th Jan.. 1936.	2nd 1936. Feb.,	7 „
5	Hen . .		Do. .	Do. .	Do. .	7th 1936. Feb.,	III and was des- troyed.
6	Hen . .		Do. .	Do. .	Do. .	4th 1936. Feb.,	9 days.
7	Hen . .	24 hours.	25th Jan., 1936.	26th Jan., 1936.		29th 1936. Jan.,	3
8	Cook . .		Do. .	Do. .		30th 1936. Jan.,	4 „
9	Hen . .		Do. .	Do. .		31st 1936. Jan.,	5 .,
10	Hen . .		Do. .	Nil .	26th Jan., 1936.	2nd 1936. Feb.,	7 „
11	Cock . .		Do. .	Do. .	Do. .	4th 1936. Feb.,	9 „
12	Cock . .		Do. .	Do. .	Do. .	4th 1936. Feb.,	9 „
13	Cock . .	Simulta- neously.	26th Jan., 1936.	26th Jan., 1936.		29th 1936. Jan.,	3 „
14	Cock . .		Do. .	Do. .		30th 1936. Jan.,	4 „
15	Cockrel .		27th Jan., 1936.	Nil .	27th Jan., 1936.	2nd 1936. Feb.,	7 „
16	Cockrel .		Do. .	Do. .	Do. .	7th 1936. Feb.,	III and was des- troyed.
17	Pullet . .	Controls .	Nil .	26th Jan., 1936.		31st 1936. Jan.,	5 days.
18	Pullet . .		Do. .	Do. .		29th 1936. Jan.,	3 „
19	Pullet . .		Do. .		26th Jan., 1936.	2nd 1936. Feb.,	7 „
20	Pullet . .		Do. .		Do. .	31st 1936. Jan.,	5

All fowls got affected with the disease and died within twelve days except
two which too were ill and were, therefore, destroyed to wind up the experiment
in order to enable me to attend to urgent duties awaiting elsewhere. These
results show that trypanblue failed to protect the birds when they were infected
artificially or exposed to heavy infection under artificial conditions.

Prophylactic value of Trypanblue under field conditions

Experiment 2.—This experiment was carried out at Kedgaon, a village in
Poona District. The disease had broken out there on 20th December 1935 and
had inflicted a heavy loss to the village as more than 300 fowls had died. The
experiment was undertaken on the fowls which were located in one street where
the statistics were 88 attacks, 87 deaths and one " still affected ". Two hundred