V. R. RAJAGOPALAN 35
TABLE I
Phenol-agar heated antigens |
Source Nat. Inst. Type Culture No. |
Heat-stabile Somatic Factors |
Titre |
|
4 hours |
24 hours |
|||
S. paratyphi-A . |
13 . . |
I, II . . . |
Nil |
Nil |
S. paralyphi-B . |
15 . . |
IV, V (7, 8) . . |
Nil |
200 |
S. abortus-equi . |
766 . . |
IV (7, 8) . . |
400 |
800 |
S. paratyphi-C . |
90 . . |
VI, VII . . |
Nil |
Nil |
S. Stanley . . |
92 . . |
IV, V (7, 8) . . |
50 |
200 |
S. reading . . |
72 . . |
IV (7, 8) . . |
Nil |
100 |
S. derby . . . |
1,729 . . |
IV (7, 8) . . |
200 |
400 |
S. typhi . . . |
786 . . |
IX (8) . . . |
*800 |
*800 |
S. enteritidis† . . |
410 . . |
IX (8) . . . |
Nil |
100 |
S. pullorum . . |
969 . . |
IX . . . |
*800 |
*800 |
S. gallinarum . . |
416 . . |
IX . . . |
*800 |
*800 |
S. anatum . . |
Local Strain . |
X, III . . . |
Nil |
Nil |
S. newport . . |
129 . . |
VI, VIII (7) . . |
Nil |
100 |
Shirlaw's organism . |
Isolated from |
? |
400 |
*800 |
* End titre not determined.
† This culture which has been in subculture in this laboratory for the last fifteen
years has since been found, by appropriate tests, to be in the " O " and " R " form.
(The Roman figures represent the somatic antigenic structures suggested by the
Salmonella Sub-Committee of the International Society for Microbiology, and the Arabic
figures within brackets represent additional somatic factors recognised by Bruce-
White during 1926.)
The tests were repeated with some of the organisms in which the end
titre had not been reached with a view to find out the end titre. The results
were as follows :—
S. abortus-equi . . . . . . . . |
End titre 6,400 |
S. typhi . . . . . . . . . |
„ 6,400 |
S. gallinarum . . . . . . . . |
„ 6,400 |
The above tests show that the somatic antigen of the organism under
study is remarkably related to that of S. pullorum, S. typhi, S. gallinarum
and S. abortus-equi and to a lesser extent to S. Stanley, S. derby, S. reading,
S. paratyphi-B, S. newport and S. enteritidis " 410 "
The strong relationship to pullorum and gallinarum, organisms which
possess each a single somatic factor namely IX of the Kauffmann-White
schema, strongly suggests that factor IX is at least one of the components
of the somatic antigen of the Shirlaw's organism. It is also remarkably
related to S. abortus-equi. The only somatic antigenic factor that it con-
tains, according to the Kauffmann-White schema, is factor IV. The follow-
ing absorption test was, therefore, conducted to verify if factor IV was also
concerned in this organism.