104                      Goat-Virus Vaccination in Cattle

                                      SUBJECTS

Four out of eighty-two animals which were vaccinated with goat-virus
on 1st December, 1932, at the Dairy of the Ramabai Mukti Mission
(American), Kedgaon, were selected for the test. They were the following :—
        Bullock, Paithan, 9½ years.
        Buffalo-cow, Gujar, 8½ years, pregnant 2 months.
        Cow, Gawali, 10 years, pregnant 8 months.
        Buffalo-cow, Shobha, 10 years, milching.

As regards the reaction induced by goat-virus vaccination five years ago
in these animals, fever, dullness, congested mucous membrane were common
in all; buffalo-cow, Gujar, had passed very soft faeces but had fed well, all
others had impaired appetite and buffalo-cow, Shobha, had shooting diarrhoea.
They were in good condition at the time of subjecting to the immunity test.

                        VIRULENT RINDERPEST VIRUS

The test was carried out by using virulent rinderpest virus with a view
to give a severe trial to the immunity acquired by the animals and for this
purpose a consignment of bull-virus from the Imperial Veterinary Research
Institute, Mukteswar, was obtained. The virus was collected there on 1st
December, 1937, and was received at Kedgaon by post on 5th December, 1937,
It was received in an excellent condition of preservation.

                                        CONTROLS

Two bulls, one two years of age and another three and half years old,
which were known to have not suffered from rinderpest previously, were bought
at Yevat and transported to Kedgaon, covering a distance of six miles. They
were quite healthy and their body condition was fairly good.

                                    IMMUNITY TEST

The four vaccinated animals and the two controls were injected, each
with 2 c.c. of the bull-virus, on 5th December, 1937, and they were housed in
a segregation camp specially arranged. They were kept under observation up
to 21st December, 1937, i.e., for sixteen days, during which period the reaction
produced in individual animals was carefully noted and recorded in the tem-
perature charts (Figs. 1 to 6). A summary of the same is given in Table I.