210 Studies on a Natural Outbreak of Pigeon-Pox
COMPLEMENT FIXATION TESTS
Sera from natural cases of pigeon-pox and pigeons after artificial infection
with the English strain were tested for the presence of complement fixing
bodies. The tests were controlled by healthy sera. Kolmer's [1931] technique
of the " Wassermann test" was employed with slight modifications as required.
The results are tabulated in Table VI.
TABLE VI
Complement fixation tests
Kind of serum |
Quantity of serum |
Result with antigens |
|
Indian strain |
English strain |
||
Anti-pigeon pox (Indian) . . . |
0.1 |
+ |
— |
0.05 |
+ |
— |
|
0.01 |
— |
— |
|
0.005 |
— |
— |
|
Anti-pigeon pox (English) . . |
0.1 |
— |
— |
0.05 |
— |
— |
|
0.01 |
— |
— |
|
0.005 |
— |
— |
|
Healthy pigeon .... |
0.1 |
— |
|
0.05 |
— |
— |
|
0.01 |
— |
— |
|
0.005 |
— |
— |
|
Healthy fowl ..... |
0.1 |
— |
— |
0.05 |
— |
— |
|
0.01 |
— |
— |
|
0.005 |
— |
— |
N.B. + = Fixation of complement (no haemolysis).
— = No fixation of complement (haemolysis).
From the above table it appears that the complement fixing bodies are
not, as a rule, demonstrable in the serum of pigeons recovered from pigeon-
pox. Hence this test cannot be applied with advantage in this disease
caused by either the English or the Indian strain of the virus.