210          Studies on a Natural Outbreak of Pigeon-Pox

                COMPLEMENT FIXATION TESTS

Sera from natural cases of pigeon-pox and pigeons after artificial infection
with the English strain were tested for the presence of complement fixing
bodies. The tests were controlled by healthy sera. Kolmer's [1931] technique
of the " Wassermann test" was employed with slight modifications as required.
The results are tabulated in Table VI.

                                    TABLE VI

                        Complement fixation tests

Kind of serum

Quantity of

serum

Result with antigens

Indian strain

English strain

Anti-pigeon pox (Indian) . . .

0.1

+

0.05

+

0.01

0.005

Anti-pigeon pox (English) . .

0.1

0.05

0.01

0.005

Healthy pigeon ....

0.1

0.05

0.01

0.005

Healthy fowl .....

0.1

0.05

0.01

0.005

N.B. + = Fixation of complement (no haemolysis).
— = No fixation of complement (haemolysis).

From the above table it appears that the complement fixing bodies are
not, as a rule, demonstrable in the serum of pigeons recovered from pigeon-
pox. Hence this test cannot be applied with advantage in this disease
caused by either the English or the Indian strain of the virus.