302         'Lahore Canine Fever' and 'Tick Fever' of dogs

normal saline from puppy No. 6, an acute experimental case (Chart No. 1),
which had been subjected to experimental treatment with S. U. P. 36.
No reaction occurred to the second inoculation but on 11th August
1935, the picture of an early commencing secondary anaemia was obtained
in blood film examination. The puppy was then destroyed by electrocu-
tion. No parasites had been observed in its peripheral blood. The weight
of this puppy was 22.5 lb. and at post mortem the spleen weighed six ounces
(i.e., 1.66 per cent of the body weight). According to Sisson, the weight of
the spleen in a normal sized dog—it is presumed that this is about
20 lb.—is 1½ to 2 oz. Accepting the lower figure 1½ oz. the normal percentage
of the spleen to the body weight is, therefore, in the region of 0.47 per cent.
The spleen of puppy No. 13 (Chart No. 5) was, therefore, according to these
figures, increased in weight by three and a half times the normal.

The liver weighed 1 lb. 12 oz., i.e., 7. 6 per cent of the body weight. It is
stated that, in health, the liver of the dog weighs approximately 3 per cent of
the body weight.

It will be seen then that, in spite of the failure to react to the inoculation of
virulent blood, there was a terminal secondary anaemia, co-ordinated with a
decided splenic and hepatic enlargement. The important observation is
that, in microscopic examination, the cellular reaction of the spleen and
liver was similar to that encountered in ' Lahore Canine Fever ', although
not so pronounced in degree.

Puppy No. 15 (Chart 6) was a somewhat similar case. It was inocu-
lated on 30th July 1935 with 5 c. c. of spleen emulsion in normal saline
from Puppy No. 12 (Chart 4), and observations were continued over
seventy-one days. The temperature remained practically normal over
the first month and no parasites were observed in daily examination, nor were
there any pathological alterations in the blood. The temperature over the
second month ran only one to two degrees, higher than in the previous month,
and on the forty-fifth day was 103.6°F. On the forty-ninth day, decided
anaemic changes commenced to show in the blood and these were strongly
progressive. P. gibsoni, in very scanty numbers, averaging one to two per
field, made its appearance on the sixty-fifth day, and was present in equally
scanty numbers until the seventy-first day, when the puppy died.

The weight of this dog was 21½ lb. At post mortem, the spleen weighed
eleven ounces, i.e., the percentage weight of the spleen was 3.2 per cent of the
body weight, or more than seven times the normal. The liver weighed 1 lb.
4 oz. or 5.3 per cent of the b ody weight, i.e., nearly twice the normal weight.
The spleen in this case was livid in colour, the pulp was soft in consistence and
haemorrhagic infarcts were noted. The liver was fatty and distinct acute
interstitial myocarditis was present, with areas of patchy necrosis of the mus-
cular fibres. Histopathological lesions similar to those of 'Lahore Canine
Fever ' were found in the spleen and bone marrow. The liver was not affected
to such a marked degree.