S. GANAPATHY IYER                              381

for other bird infections and separate attendants are provided. Thus, there
was practically no possibility of accidental Doyle's disease infection in this
shed. Drooping of wings with marked paralysis of the legs was the symptom
in the pigeon which attracted attention, while harvesting pox virus crusts,
and led to a suspicion that Doyle's disease might be the cause. There was
a whitish diarrhoea soiling the tail feathers. In order to determine the speci-
fic cause of the paralysis, the bird was destroyed the same day that the ner-
vous symptoms were observed.

The post-mortem examination revealed intestinal congestion and slight
spleenic enlargement. From the heart blood a pure culture of Escherichiacoli
was isolated. In addition, the films showed Haemoproteus, a frequent
finding in the experimental birds at this Institute. The autopsy revealed
nothing unusual otherwise. The whole spleen was removed with aseptic
precautions, emulsified in 10 c. c. of normal saline solution, and filtered
through a Seitz filter. A healthy fowl was injected subcutaneously with the
whole filtrate. This fowl reacted (Doyle's disease) and died.

                                            TABLE I

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