162               Contagious Pleuro-Pneumonia of Goats

Experiment 24.— The naso-pharynx after intra-nasal insufflation

A goat was given an insufflation with 10 c. c. of 1 per cent aqueous methy-
lene blue by the usual technique. The animal was immediately destroyed,
and the naso-pharynx together with the larynx and trachea examined by
medial section. Almost the entire nasal cavity, including the surface of the
turbinates and much of the ethmoid, and the upper part of the pharynx, were
deeply stained with the dye. A very few drops of the dye were evident on the
mucous membranes and vocal chords in the larynx, but no extension to the
tracheal mucosa could be discerned. The experiment suggests that the disease
is transmitted by absorption through the naso-pharyngeal mucous membrane,
and probably from there via the lymphatics.

Experiment 25.— Filtrability of the agent through the Seitz filter

An experiment on this issue was made with the pneumonic lung emulsion
used for the 1st test insufflation in Experiment 32, and which infected four of
the six controls in that experiment (Table XLIV). The preparation in ques-
tion was 1 : 1 Tyrode pneumonic lung emulsion which was mixed with an
equal volume of plain peptone broth, filtered at 12 cm. negative pressure
through the Seitz E. K. disk, and administered intra-nasally in 15 c. c. doses to
four goats. Two controls received the unfiltered residual fluid in the filter
chamber (pneumonic lung emulsion+broth) in the same dosage. The results
are shown below :

                                                              TABLE XXVI

It will be seen that both the controls reacted, one dying of pneumonia at the
15th day p. ins., the other being destroyed in extremis and found positive on
the same day. Two goats of the experimental group unfortunately died of
other causes on the 5th and 6th days p. ins., too early for the appearance of
lesions. One other, No. 332, died on the 29th of August 1939, 20 days p. ins.
and the typical pneumonia with pleurisy and effusion was found at autopsy.
The remaining goat, slaughtered on the following day, was negative.

Summary.—One of the two goats insufflated with a Seitz filtrate of a
virulent pneumonic lung emulsion, died with typical pneumonia 20 days
p. ins.