K. S. NAIR, R. KRISHNAMURTI & G. S. KALYANASUNDARAM         313

                                              TABLE III

To determine the viability at room temperature of dried goat spleen vaccine—
                                    Batch No. III of Madras

                                        Dose—0.0025 gm.

Serial
No.

Period
of
storage

Reaction
to
vaccination

Reaction
after
retest

Interval
between
vaccination
and
retest

Remarks

1

2

3

4

5

6

Days

1

Nil . .

1. Mild

Nil . .

18

2. Moderate

Nil . .

21

2

Nil . .

1. Mild

Nil . .

15

2. Mild

Nil . .

18

3

1 day .

1. Moderate

Nil . .

19

2. Severe and
died.

. .

..

4

2 days .

1. Mild

Nil . .

16

2. Nil

Mild . .

12

5

3 days .

1. Moderate

Nil . .

17

2. Moderate
(late).

Nil . .

27

6

4 days .

1. Nil .

Mild . .

10

2. Nil .

Nil . .

10

7

5 days .

1. Nil .

Severe and
died.

13

2. Moderate*

Nil

28

* Probably a
case of
natural in-
fection.

8

6 days .

1. Nil . .

Severe .

12

2. Nil. .

Mild . .

9

9

7 days .

1. Nil . .

Mild . .

8

2. Nil . .

Nil . .

8

Note:
Two calves were used for each serial number.
In serial numbers two to nine, the vaccine was kept in thermos jar with ice packing
for three days and then kept at room temperature for the number of days specified
against each.