43

The pericardial cavity contains an excess of fluid in
which fibrin may be present. Myocarditis is generally found
and endocarditis is an almost diagnostic post-mortem symp-
tom.

The spleen and blood are of normal appearance. The
bladder is inflamed and the urine darker coloured than normal.

On microscopical examination of the heart wall, micros-
copic hæmorrhages are seen between the muscle fibres and
under an oil immersion lens large clusters of bacilli are found.

The former appearance is well seen when Weigert's Iron
Hæmatoxylin followed by Van Gieson's stain are used, and
the bacilli are best seen after 24 hours staining with
Lœffler's Alkaline-Methylene-Blue.

DISTRIBUTION OF THE BACILLI IN THE ANIMAL BODY.

They are present in every organ and fluid. In all animals
one can generally obtain cultures easily from the heart-blood.

The best and most abundant growth is obtained from
the liver and spleen. Less abundant growths are obtained
from the mesenteric glands, subcutaneous tissues, peritoneal,
pleural and pericardial fluids, testicles and kidneys.

The urine taken from the bladder on post-mortem
examination was always found infective, and judging by the
thickness of the growth on agar, the bacilli are very plentiful
in this fluid.

In inflammatory and hæmorrhagic patches in the tissues
clumps of bacilli are seen on section.

                  INOCULATION ANIMALS.

                                 Buffaloes.

A buffalo calf was inoculated with .5 c.c. emulsion of
H. S. culture 24 hours old from a rabbit which died in 13½
hours.

The buffalo died in 21 hours.

The symptoms were those of acute intoxication, the
animal being in a deep stupor.

He lay huddled up in a corner of the stable with his
head turned round on the flank and the breathing laboured
as if in pain.

Diarrhœa and dysentery set in.