47

day the swelling had slightly decreased and she was feeding
well.

On the 4th day swelling still less but painful ; 6th day
swelling disappeared and sheep well.

On the 7th day was reinoculated with 1½ c.c. agar
culture emulsion from a. rabbit which died in 22½ hours.

A local swelling appeared, but the sheep's general
health did not suffer.

Two days later the swelling had gone.

On the 13th day reinoculated with 3 c.c. culture emul-
sion, and only a slight local reaction resulted.

Three days later some blood was drawn off and clotted
and the serum when injected into rabbits proved fatal.

On the 22nd day sheep was reinoculated with 10 c.c.
culture emulsion and again there was a local reaction.

On the 39th day death resulted.

Sheep No. 50 was inoculated subcutaneously with 2 c.c.
H. S. bouillon culture.

There was a severe local reaction and for a few days the
sheep was dull and off feed.

For about three weeks there was intermittent high
fever, the temperature rising to 105°F. ; then the sheep
became quite well again. The reaction to inoculation was
very severe, but it left behind a powerful immunity as
proved by subsequent inoculations with large doses of
culture giving practically no reaction, whereas the control
rabbits died.

Sheep No. 53 was inoculated subcutaneously with
10 c.c. H. S. bouillon cult e and died in 40 hours. There
was an extensive local reaction and the temperature rose
to 106°F.

The bacillus was obtained from the post-mortem
examination.

                                GOATS.

Goat No. 19 was inoculated subcutaneously with .5 c.c.
bouillon culture 24 hours old.

The second day the temperature rose to 104°F. and on
the 5th, 6th and 7th days nearly reached 105°F., then went