210    PREPARATION OF ANTI-RINDERPEST SERUM.

The lungs showed extravasation.

The heart presented petechiæ on the endocardium.

The blood was dark and did not coagulate readily.

                     CAUSE OF DEATH.

Ruediger does not mention having had any fatal results
after the injection of artificial peritoneal fluid.

Baldrey in the Journal of Tropical Veterinary Science, Vol.
VI, No. 1, 1911, attributes the deaths to Rinderpest toxin,
but in our present state of knowledge regarding Rinderpest,
this theory cannot be accepted until further proof is forthcom-
ing, especially as under certain circumstances, the injection
of normal blood produces somewhat similar results. The
matter is at present being investigated by the Imperial Bacterio-
logist, and the result of his experiments will shortly be pub-
lished.

PREPARATION OF SERUM BY ARTIFICIAL PERITONEAL FLUID

      FOR COMPARISON WITH SERUM PREPARED BY VIRULENT

                                 BLOOD INJECTION.

The amounts of artificial peritoneal fluid that could be
safely injected having been determined by the previous experi-
ments, several animals were immunised by the simultaneous
method, of these animals half were hyperimmunised by artifi-
cial peritoneal fluid and the other half by the injection of viru-
lent blood.

The animals were a very fair sample of the bulls usually
obtainable for the preparation of serum, and both lots were
kept under like conditions.

Those hyperimmunised by artificial peritoneal fluid receiv-
ed injections at the rate of 700 c.c. per every 100lbs. body
weight, and those hyperimmunised by virulent blood receiv-
ed injections at the rate of 1,000 c.c. per 100lbs. body weight.

All injections were well borne and rapidly absorbed.

The animals were bled for serum 21 days after in-
jection.