PART III.

               HÆMORRHAGIC SEPTICÆMIA.

FURTHER TESTINGS OF THE HÆMORRHAGIC SEP-
          TICÆMIA ANTI-SERUM AND VACCINE.*

                                       BY

MAJOR J, D. E. HOLMES, M.R.C.V.S., M.A., D.Sc., I.C.V.D.,

         Imperial Bacteriologist to the Govt. of India.

In a previous publication (C. V. D. Memoirs, No. 1), I
described the methods of preparation of a serum and dead
vaccine against Hæmorrhagie Septicæmia of Bovines. The
results of the experimental tests carried out in the Laboratory
shewed that the serum in a dose of 5 to 10 c.c. protects cattle
against a lethal dose of virus inoculated 24 to 48 hours after
the serum and that an injection of 25 c.c. serum gives a pro-
tection for a period of about four weeks. The vaccine in a
dose of 5 c.c. protects against a lethal dose of virus inoculated
four days after the injection of the vaccine. The immunity
following a dose of vaccine in amounts of 5 to 10 c.c. persists
for a period of about six weeks.

During the past two years, 1,825 doses of this Anti-Serum
and 63,810 doses of the Vaccine have been issued for use in
the field, and as far as it was possible to trace, the results have
been satisfactory. The Superintendent, Civil Veterinary
Department, C. P., in his letter No. 343-20, dated 11th February

                              * Results arrived at in July 1911.