270 ANTHRAX.
The serum in doses from 25 to 100 c.c. protects country-
bred ponies, mules and donkeys against the virus inoculated
simultaneously on the opposite side. An injection of 100 c.c.
protected two Australian horses out of three.
SERUM, PLUS VIRUS 24 HOURS AFTER. RE-TESTED AFTER
SIX WEEKS.
|
No. |
Serum. |
Virus. |
Result, |
Re-tested |
Result. |
|
|
c.c. |
c.c. |
|||||
|
Pony |
1 |
25 |
0.5 |
Lived. Temp. re- |
42 |
Lived. No reaction. |
|
„ |
2 |
25 |
0.5 |
„ No reaction |
42 |
„ „ „ |
|
„ |
3 |
25 |
0.5 |
„ „ „ |
42 |
„ „ „ |
|
„ |
4 |
25 |
0.5 |
„ „ „ |
42 |
„ „ „ |
|
Donkey |
5 |
25 |
0.5 |
„ „ „ |
42 |
„ „ „ |
An injection of 25 c.c. serum protected four country-bred
ponies and one donkey against the virus inoculated 24 hours
after the serum. These animals were immune when re-tested
six weeks after the first inoculation.
SERTUM, PLUS VIRUS AFTER FIVE WEEKS.
|
No. |
Serum. |
Virus. |
Result. |
||
|
Pony |
1 |
50 c.c. |
0.5 c.c |
Lived. |
Temperature reaction. |
|
„ |
2 |
50 „ |
0.5 „ |
„ |
„ „ |
|
„ |
3 |
50 „ |
0.5 „ |
„ |
No reaction. |
|
„ |
4 |
50 „ |
0.5 „ |
Died in 4 days. |
|
|
„ |
5 |
50 „ |
0.5 „ |
Lived. |
No reaction. |
|
Donkey |
6 |
50 „ |
0.5 „ |
„ |
„ „ |
An injection of 50 c.c. serum protected four country-bred
ponies out of five and one donkey against the virus inoculated
five weeks after the first serum injection
CONCLUSIONS.
I. A potent Anti-Anthrax Serum can be prepared from
Cattle, Sheep and Horses.
II. The serum prepared by injections of 48 hours broth
cultures, to which was added an emulsion of Anthrax bacilli
from Agar surface growth, gave best results.