10           REPORT OF THE CIVIL VETERINARY DEPARTMENT, BURMA,

Immunizing value of dried goat spleen vaccine after storage for 30
days at room temperature.—Two Kyaukpyu cattle were each given 0.5
grm. of dried goat spleen after storage for 30 days at room temperature.
Both developed a mild thermal reaction and a very severe temperature
reaction to virulent bull virus given 30 days later. Both controls died.
This also indicates that a big dose of vaccine attenuated by storage
confers some protection whereas a small dose does not.

(e)  Immunizing value of Desiccated Goat Spleen Vaccine stored at 37°C.
and at room temperature for 12 hours alternately.—The vaccine was
tested after storage for six, eight, ten and twelve days, two Kyaukpyu
cattle being used on each occasion, each animal being given 0'0025
grm. Previous experiments had shown that 0.005 grm. of the vaccine
immunizes after storage for three days at 37°C. This experiment shows
that six days' storage at 37°C. and at room temperature for twelve hours
alternately destroys the immunizing properties of the vaccine, indicgting
that the attenuation which commences at 37°C. is not arrested by
subsequent storage at a lower temperature.

(f)  The Immunization of Buffaloes.—A small experiment was done on
15 buffaloes to determine the effect of the quantity of Rinderpest Desic-
cated Goat Spleen Vaccine on the severity of the reaction. The
buffaloes were about 18 months to 3 years old. They were in fairly
good condition when given the vaccine but lost condition fairly rapidly
while under experiment. The following conclusions were drawn :—

(1)  The reaction is not dependent upon the quantity of the vaccine

(2)    very small dose of vaccine may provoke a reaction but may
give insufficient protection.

13, 0.00025 grm. of dried spleen powder protects buffaloes against
2.5 c.c. virulent blood.

It is proposed to do further work on older buffaloes to confirm these
findings and to determine the cause of the rapid loss of condition after
inoculation.

(g)  Bennett's Glycerine-water Vaccine.—Bennett showed that a glyce-
rine water emulsion of spleen of a bovine killed at the height of reaction
to virulent Rinderpest is attenuated by storage, and that it may safely be
used as a vaccine after three days at 37°C., and remains effective for
30 days at this temperature. It was thought that this vaccine would be
useful in certain areas in Burma. Vaccine prepared by Bennett's
method was given to Kyaukpyu cattle, weighing about 200 lbs., in
quantities equivalent to one grm. of spleen pulp. The vaccine was
used after storage for 4, 6, 15 and 20 days at 37°C. two Kyaukpyu cattle
being used on each occasion. All were subsequently given virulent
blood on the same day, so that the interval between the injection of the
vaccine and the injection of the virulent blood varied, being 27 days
for the 4-day group, 25 days for the 6-day group, 20 days for the 15-day
group and 11 days for the 20-day group. Both animals in the 20-day
group died ; one in each of the other groups died, the other