( 13 )

From lymph of calf No. 14 vaccinated calves Nos. 17 and 19. Also, sent 4 tubes to Akola ;
used on the 4th day. Result—successful. Four tubes to Buldana, used on the 7th day.
Result—unsuccessful.                                             —

Vaccinated 10 children with 4 scabs 6 days old. Result—2 successful of 3 scars each.

Vaccinated 7 children with 3 scabs 8 days old. Result—1 successful of one scar.

Vaccinated 12 children direct from calf. Result—all successful; very good.

One scab given to Vaccinator Ganeshpersad, who vaccinated 3 children on 11th day.
Result—«1 successful scar.

Nursia Pochia was one of the afore-mentioned 12 children with typical vesicles selected
for the " retro-vaccine " experiment.

Animal vaccination scabs take 20 days to mature and drop.

According to above experiment scabs 11 days old may be used to start a lymph supply,
Animal lymph used direct from calf to vaccinate children proves most successful.
Animal lymph on points 20 days old has proved successful.

Either needles or lancets might be used to vaccinate a calf. The pox of needle insertions
are large, contain more lymph, but are dark and not so pleasing to the eye as the pox of lan-
cet punctures. The forcep in use is more suited for lancet than needle punctures. Its use in
needle punctures causes much waste of lymph.

                                                      NOTE.

A policeman, age 25, came in from Chikhli to the Civil Hospital, Buldana. He said he
had fever on the 2nd, 3rd, and 4th December 1888, and that an eruption appeared on the
morning of the 5th December 1888 ; when admitted on the 6th morning, the 5th day of illness.
he had a well-defined eruption of discrete small-pox.

On the morning of 8th December, 7th day of illness, 4th day of appearance of eruption,
virus was taken with every precaution that vaccine lymph was not on the lancets and inserted
in 45 punctures on the teats of a calf about 10 months old. I was careful to select a calf
from among my cattle, so that I could be satisfied that the animal had not suffered from foot
or mouth disease.

On the morning of the 9th December the lymph in the vesicles on the man's body was
found to be opaque and was rejected. On the 13th December, or 6th day after inoculation.
there were numerous well-developed vesicles on the teals of the calf, and lymph was transferred
to punctures on the teats of another calf with the same precautions.

On the 18th December the result of the inoculation of the second calf was satisfactory,
and a third calf was similarly operated upon.

On 23rd December other two calves were inoculated—one sent to Sanitary Commissioner,
the other taken by the Circle Vaccinator to Amrapur Circle to be used there in vaccinating
children. The results there will be reported to Sanitary Commissioner by the Native Super-
intendent of Vaccination.

On the same day, 23rd December, lymph taken from the third calf was used to vaccinate
eight children in the bazaar; three of these operations proved successful, the course of the disease
being as usual, except that there was more decided fever on the 4th and 5th days and the
arcola was deeper coloured and larger. The other five children were operated on again with
lymph from the arms of the three successful cases, and the result was success. Since then the
lymph has been used from arm to arm, cases proving successful in every case.

The scars left on the arms of the first cases on examination with a lens present the charac-
teristic appearance of a vaccine scar.

One of the first eight children operated on I found on inspection had been vaccinated suc-
cessfully two years before and had two good scars on the left arm. The operation on the right
arm on the 23rd December failed, but on repetition on 28th proved successful, and the lymph
is still in use in continuing vaccine operations.

                                                                        (Signed) D. CALLAGHAN

                                                                              Civil Surgeon, Buldana.